Over the past decades, greenhouse gas emissions have grown exponentially, exacerbating the climate crises. This research aims to contribute to the development of green fuels that do not produce greenhouse gases by advancing bio-photosynthetic production of hydrogen. This study focuses on two genetically modified mutants of Chlamydomonas reinhardtii (C.reinhardtii) designed to alter the photosynthetic system. The first mutant overexpresses the Rieske subunit, and was hypothesized to enhance photosynthesis efficiency and boost hydrogen production. The second mutant fuses the Hydrogenase enzyme (HydA) with an antibody targeting cytochrome b6f,aiming to strategically position the enzyme and disrupt electron flow. The first mutant was measured for Non-Photochemical Quenching (NPQ), while the second mutant was measured for hydrogen production. Results indicate that the NPQ levels in the mutant were lower than in the wild type (WT), suggesting increased photosynthetic efficiency. Hydrogen production in the second mutant displayed a more linear trend compared to WT, potentially indicating enhanced hydrogen output over time. These findings suggest that modifying the photosynthetic system of C. reinhardtii could improve hydrogen production efficiency, offering promising advancements for green energy technologies.